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KMID : 1007520160250051437
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Food Science and Biotechnology
2016 Volume.25 No. 5 p.1437 ~ p.1442
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Linarin down-regulates phagocytosis, pro-inflammatory cytokine production, and activation marker expression in RAW264.7 macrophages
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Kim Bo-Mi
Lee Jong-Hun
Seo Myung-Ji
Eom Seok-Hyun
Kim Woo-Ki
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Abstract
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Plant-extracted flavonoid glycosides have been reported to be bioactive compounds with pleiotropic functions, including antioxidant, anti-inflammatory, and anti-cancer effects. This study investigated the anti-inflammatory role of linarin (acacetin-7-rutinoside, which is found in Chrysanthemum indicum (Gam-Guk) and Dendranthema zawadskii (Gu-Jul-Cho)), on lipopolysaccharide-stimulated RAW264.7 macrophages. Linarin treatments exhibited no cytotoxicity up to a concentration of 30 μM, as assessed by MTT assay. The production of nitric oxide, an inflammatory mediator, was decreased by addition of linarin. The secretion of pro-inflammatory cytokines, interleukin-1β and interleukin-6, was significantly decreased in a dose-dependent manner. Linarin also decreased the phagocytic ability of macrophages following co-culture with fluorescent beads. In addition, expression levels of antigen-presenting surface markers, MHC II and CD80, were suppressed by linarin. Taken together, these results indicate that the flavonoid glycoside linarin has an anti-inflammatory effect, in part through the suppression of phagocytosis, cytokine production, and antigen presentation in macrophages.
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KEYWORD
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flavonoid, inflammation, cytokine, phagocytosis, antigen-presentation
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